Protein isoelectric point calculator



Isoelectric point, the pH at which a particular molecule carries no net electrical charge, is an important parameter for molecular biology analysis, especially in 2D gel electrophoresis (2D-PAGE), isoelectric focusing and liquid chromatography–mass spectrometry (LC-MS)

Below is the text area where you can paste amino acid sequence of analyzed protein (peptide) or multiple sequences in fasta format



Note:
  • Input should be ONE sequence in plain text or MULTIPLE sequences in FASTA format (then limit is set to 50,000 chars)
  • Input can be upper or lower case.
  • Input should be in one letter amino acid code.
  • Alphabet allowed: VXCDBFMOLNYIQTGHWESKPAUR.
  • All non-amino acid characters will be removed from the sequence.
  • For big datasets use standalone version or split your input into 50k chunks.
Input example: for Ala-Pro-Lys-His-Ala-Tyr peptide, please enter APKHAY

Standalone version of the program (available after IPC publication acceptance)
IPC in Python
(command line, any OS)
IPC with GUI
(Windows)

This site had been cited > 100 times (according Google Scholar)

Contact: Lukasz P. Kozlowski