Protein isoelectric point calculator



Isoelectric point, the pH at which a particular molecule carries no net electrical charge, is an critical parameter for many analytical biochemistry and proteomics techniques, especially for 2D gel electrophoresis (2D-PAGE), capillary isoelectric focusing (cIEF) and liquid chromatography–mass spectrometry (LC-MS)

Below is the text area where you can paste amino acid sequence of analyzed protein (peptide) or multiple sequences in fasta format



Note:
  • Input should be ONE sequence in plain text or MULTIPLE sequences in FASTA format (limit is set to 50,000 chars)
  • Input can be upper or lower case.
  • Input should be in one letter amino acid code.
  • Alphabet allowed: VXCDBFMOLNYIQTGHWESKPAUR.
  • All non-amino acid characters will be removed from the sequence.
  • For big datasets use standalone version or split your input into 50k chunks.
Input example: for Ala-Pro-Lys-His-Ala-Tyr peptide, please enter APKHAY

Standalone version of the program
IPC in Python
(command line, any OS)


Reference: Kozlowski LP (2016) IPC - Isoelectric Point Calculator http://dx.doi.org/10.1101/049841

This site had been cited > 100 times (according Google Scholar)

IPC is available only for non-commercial and academic users, for details see license

Contact: Lukasz P. Kozlowski