 Polish version |
Lukasz
Kozlowski
Kielce, Poland 2007
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Calculation of protein isoelectric point
| Welcome,
here is the place were you can online estimate protein isoelectric point.
It is higly important to know this parametr, even approximately, during
molecular biology analyses, especially in 2D gel electrophoresis
and isoelectric focusing.
Additionally, there was presented short protein isoelectric point
calculation manual. You can learn how to write a computer program which
will estimate isoelectric point or simply download ready one.
Presented
C++ program make use of bisection
method, one of the most widely used numerical algorithms. |
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Below is the text area where you can paste one, raw amino acid sequence of analyzed protein (peptide) |
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Choose appropriate pK values model
Note: the sequence should be in UPPERCASE LETTERS (lowercase letters and meaningless uppercase leters will be ignored)
Input example:
If you would like to analyze Ala-Pro-Lys-His-Ala-Tyr peptide, please enter APKHAY
Additionally, you can choose between seven different sets of pK values (for more information see a table).
